UnivTrevesV1, Main, Exploration, bibRecord, 001C57

Determination of free DNA in soils

Identifieur interne : 001C57 ( Main/Exploration ); précédent : 001C56; suivant : 001C58

Determination of free DNA in soils

Auteurs : Jürgen Niemeyer [Allemagne] ; Frank Gessler [Allemagne]

Source :

Journal of Plant Nutrition and Soil Science [ 1436-8730 ] ; 2002-04.

RBID : ISTEX:F7C00836578BCC7AA64F1E617900A5617B4C0240

Descripteurs français

Pascal (Inist)
- ANALYSE DE SOL, Analyse quantitative, Centrifugation, Composé fluorescent, Concentration chimique, DNA, DNA bicaténaire, Distribution concentration, Détermination, Etude expérimentale, Etude méthode, Extracellulaire, Fertilisation minérale, Fertilisation organique, Forme libre, Horizon sol, Luvisol, Lysimètre, Lysimétrie, Marquage fluorescent, Mesure au laboratoire, Mesure concentration, Occupation sol, PicoGreen, Podzol, Procédé séparation, Profil profondeur, Profil pédologique, Protocole expérimental, Réactif analytique, Sol minéral, Solution édaphique, Spectrométrie fluorescence, Type de sol, Ultrafiltration.
Wicri :
- topic : Analyse quantitative, Type de sol.

English descriptors

KwdEn :
- Chemical concentration, Concentration distribution, Concentration measurement, DNA, Determination, Experimental study, Fluorescence spectrometry, Fluorescent labelling, Free form, Laboratory measurement, Luvisol, Method study, Mineral soils, PicoGreen, Podzol, SOIL TESTING, free DNA, lysimeter, soil.

Abstract

The concentration of free DNA in soils has to be determined in order to understand the fate and the transport of extracellular DNA. A protocol for the extraction and determination of free DNA was developed. The procedure uses separation steps, i.e. centrifugation and ultra‐filtration. The free dsDNA was stained with PicoGreen® and determined fluorimetrically. Samples from different soils, different soil horizons, soil waters and under different land use systems were analyzed. It was found that in nearly all samples free DNA was detectable. Free DNA concentrations of up to 1950 ng (g dried sample)—1 could be detected depending on depth, soil type and system of land use.

Url:

https://api.istex.fr/document/F7C00836578BCC7AA64F1E617900A5617B4C0240/fulltext/pdf

DOI: 10.1002/1522-2624(200204)165:2<121::AID-JPLN1111121>3.0.CO;2-X

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">The concentration of free DNA in soils has to be determined in order to understand the fate and the transport of extracellular DNA. A protocol for the extraction and determination of free DNA was developed. The procedure uses separation steps, i.e. centrifugation and ultra‐filtration. The free dsDNA was stained with PicoGreen® and determined fluorimetrically. Samples from different soils, different soil horizons, soil waters and under different land use systems were analyzed. It was found that in nearly all samples free DNA was detectable. Free DNA concentrations of up to 1950 ng (g dried sample)—1 could be detected depending on depth, soil type and system of land use.</div>
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Determination of free DNA in soils

Determination of free DNA in soils

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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